Becoming the Gothic Archive: From Digital Collection to Digital Humanities

The Gothic Archive is the flagship digital humanities project for the Marquette University library. The project was birthed from a simple digital collection, and through the partnership of faculty and librarians, was transformed into something more. The core tenets of digital collection creation were adhered to in order to create a solid foundation upon which to build the Archive. The expertise of both groups and communication were key in the evolution of the collection, and in discovering and highlighting the relationships between the objects. This case study reviews the steps Marquette took in creating the collection and taking it to the level of digital humanities project

Climate change mitigation by Greater Adelaide councils

This paper reports on carbon mitigation actions adopted by Greater Adelaide councils (n=14) in South Australia. A survey of environmental officers profiled carbon mitigation actions, emissions auditing, and motives for emissions reduction by councils. The main reasons for carbon actions were a climate change plan, showing climate leadership, and cost savings

The Turk and the Yankee: A Cross-Cultural Comparison between Turkish and American Managers

With Turkey’s developing role as a lead nation among emerging markets, the field of cross-cultural management becomes a key contributor to the interactions between Turkish and American professionals in the workplace. This study uses models of national cultural differences based primarily on the findings of Hofstede (1984), but it also incorporates those of Trompenaars and Hampden-Turner (1998) and the GLOBE project (2002), resulting in a comparative cross-cultural management analysis. In combining both academic theory and actual international experience, this paper illustrates that, through effective cross-cultural management, profound understanding and harmony can exist between international managers

Regulation of the hrp Type III Secretion System in Pseudomonas syringae pv. tomato DC3000

Pseudomonas syringae pv. DC3000 is a gram-negative bacterium that infects the model plant Arabidopsis thaliana. Pathogenicity is achieved via secretion of effector proteins into the host cytoplasm through a Type III Secretion System (T3SS). In Ps. DC3000 the T3SS (and associated effector proteins) are dependent on HrpL for their transcription. hrpL transcription is sigma54-dependent and requires two co-dependent enhancer binding proteins, HrpR and HrpS (HrpRS), for activation. HrpRS are regulated by two hrpL-dependent proteins, HrpV and HrpG, where HrpV negatively affects HrpRS activity and HrpG relieves this repression. Here the mechanism of HrpV and HrpG’s action on HrpRS activity was tested in vivo and in vitro; and the molecular determinants of HrpV and HrpG functionality were characterised by in silico and mutational analysis. Whole-gene deletion mutants of hrpV and hrpG in Ps. DC3000 revealed complications associated with inserting marker cassettes in transcriptionally-antagonistic orientations. Truncation mutants of HrpV and HrpG showed that C-terminal helices in both proteins play a functional and/or structural role. Alanine mutants indicated a structural role for residues 108-110 in HrpV and a functional role for residues 111-113 in HrpG (NQR motif). HrpV was demonstrated to form a dimer and represses the activity of HrpRS (in vivo and in vitro) but not via inhibiting ATPase activity or destabilising open promoter complexes. HrpG was shown to destabilise the HrpV dimer (potentially via the C-terminal NQR motif). Findings from this study were used to propose a revised model of hrpL regulation in which an active dimer of HrpV binds two adjacent HrpS subunits of a HrpRS hexamer to “lock” the hexamer in an unfavourable conformation. HrpG acts by destabilising the HrpV dimer into an inactive monomeric form. Additional experiments to confirm this model include creating an artificially linked dimer of HrpV and testing its repressive action on HrpRS in the presence or absence of HrpG